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epacadostat incb024360 (Incyte corporation)

Name: epacadostat incb024360
Brand: Incyte corporation
Rating: 90 (1 reviews)

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Incyte corporation epacadostat incb024360
Epacadostat Incb024360, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epacadostat incb024360/product/Incyte corporation
Average 90 stars, based on 1 article reviews

epacadostat incb024360 - by Bioz Stars, 2026-03

90/100 stars

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Immunosuppressive molecule, cytokine, and chemokine levels in HGSC CAFs after coculture with peripheral blood mononuclear cells (PBMCs). (A) Real‐time PCR expression analysis of immunosuppressive molecules ( IDO1 , COX2 , and PD‐L1 ) in CAFs with or without activated PBMC coculture. PBMCs were stimulated with anti‐CD3 antibody (OKT3) (15 ng·mL −1 ) for 5 days. (B) Real‐time PCR expression analysis of cytokine ( IL6 , CCL2 , CCL5 , CCL7 , and CCL8 ) and chemokine ( CXCL1 , CXCL5 , CXCL8 , CXCL9 , CXCL10 , CXCL11 , and CXCL12 ) levels in CAFs. Gene expression was calculated using the 2 −ΔΔCt method, with CAFs cultured alone as the reference group (CAF = 1; log₂(1) = 0), and data are presented as log2 fold change. Each line represents an independent biological replicate (CAFs from three different patients: OV02, OV04, and OV05). Statistical significance was determined by two‐way ANOVA followed by Tukey's multiple comparison test. (* P < 0.05, ** P < 0.01; *** P < 0.001, **** P < 0.0001, and ns = not significant).

Journal: FEBS Open Bio

Article Title: Indoleamine 2,3‐dioxygenase 1 inhibition reverses cancer‐associated fibroblast‐mediated immunosuppression in high‐grade serous ovarian cancer

doi: 10.1002/2211-5463.70126

Figure Lengend Snippet: Immunosuppressive molecule, cytokine, and chemokine levels in HGSC CAFs after coculture with peripheral blood mononuclear cells (PBMCs). (A) Real‐time PCR expression analysis of immunosuppressive molecules ( IDO1 , COX2 , and PD‐L1 ) in CAFs with or without activated PBMC coculture. PBMCs were stimulated with anti‐CD3 antibody (OKT3) (15 ng·mL −1 ) for 5 days. (B) Real‐time PCR expression analysis of cytokine ( IL6 , CCL2 , CCL5 , CCL7 , and CCL8 ) and chemokine ( CXCL1 , CXCL5 , CXCL8 , CXCL9 , CXCL10 , CXCL11 , and CXCL12 ) levels in CAFs. Gene expression was calculated using the 2 −ΔΔCt method, with CAFs cultured alone as the reference group (CAF = 1; log₂(1) = 0), and data are presented as log2 fold change. Each line represents an independent biological replicate (CAFs from three different patients: OV02, OV04, and OV05). Statistical significance was determined by two‐way ANOVA followed by Tukey's multiple comparison test. (* P < 0.05, ** P < 0.01; *** P < 0.001, **** P < 0.0001, and ns = not significant).

Article Snippet: Cells were treated with serially diluted concentrations of IDO1 inhibitor (epacadostat) (MedChemExpress, Monmouth Junction, NJ, USA) at 0 μ m and at concentrations ranging from 4 μ m to 0.0078 μ m using a 2‐fold serial dilution and incubated for 5 days.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Gene Expression, Cell Culture, Comparison

IDO1 inhibition effects by epacadostat on CAF‐induced T‐cell suppression and PD‐1 levels. (A) Epacadostat cytotoxicity toward OV05CAF and CAOV3 cell lines was evaluated using water‐soluble tetrazolium salt (WST) assays. Cells were treated with different epacadostat concentrations (0–4 μ m ) for 5 days, and cell viability was measured. (B) IDO1 mRNA expression in CAFs (OV02CAF, OV04CAF, and OV05CAF) was analyzed using real‐time PCR after coculture with activated PBMCs for 5 days, with or without 500 n m epacadostat. (C–E) CFSE‐labeled PBMCs were cocultured with or without CAFs (OV01, OV02, OV04, and OV05) at a PBMC:CAF ratio of 6:1 and cultured for 5 days with OKT3 (15 ng·mL −1 ) stimulation, in the presence or absence of epacadostat (500 n m ). (C) Quantified CD4+ and CD8+ T‐cell proliferation (CFSE staining; n = 4). (D) Quantified CD8+ and CD4+ T‐cell proportions ( n = 4). (E) Quantified PD‐1 levels in CD8+ and CD4+ T cells ( n = 4). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Journal: FEBS Open Bio

Article Title: Indoleamine 2,3‐dioxygenase 1 inhibition reverses cancer‐associated fibroblast‐mediated immunosuppression in high‐grade serous ovarian cancer

doi: 10.1002/2211-5463.70126

Figure Lengend Snippet: IDO1 inhibition effects by epacadostat on CAF‐induced T‐cell suppression and PD‐1 levels. (A) Epacadostat cytotoxicity toward OV05CAF and CAOV3 cell lines was evaluated using water‐soluble tetrazolium salt (WST) assays. Cells were treated with different epacadostat concentrations (0–4 μ m ) for 5 days, and cell viability was measured. (B) IDO1 mRNA expression in CAFs (OV02CAF, OV04CAF, and OV05CAF) was analyzed using real‐time PCR after coculture with activated PBMCs for 5 days, with or without 500 n m epacadostat. (C–E) CFSE‐labeled PBMCs were cocultured with or without CAFs (OV01, OV02, OV04, and OV05) at a PBMC:CAF ratio of 6:1 and cultured for 5 days with OKT3 (15 ng·mL −1 ) stimulation, in the presence or absence of epacadostat (500 n m ). (C) Quantified CD4+ and CD8+ T‐cell proliferation (CFSE staining; n = 4). (D) Quantified CD8+ and CD4+ T‐cell proportions ( n = 4). (E) Quantified PD‐1 levels in CD8+ and CD4+ T cells ( n = 4). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Labeling, Cell Culture, Staining, Comparison

IDO1 inhibition promotes T‐cell proliferation by increasing protein kinase B (Akt) phosphorylation suppressed by CAFs. (A) Flow cytometric analysis of phosphorylated Akt (ser473) in PBMCs. Cells were activated with OKT3 (15 ng·mL −1 ) in the presence or absence of OV05 CAFs at a PBMC:CAF ratio 6:1 and with or without epacadostat (500 n m ) for 5 days. Histograms show p‐Akt expression in CD8+ and CD4+ T cells. (B) Quantified p‐Akt levels in CD8+ and CD4+ T cells from experiments using three different CAFs (OV01, OV02, and OV05). (C) Flow cytometry histograms show T‐cell proliferation (CFSE stained) with increasing concentrations of LY294002 (phosphatidylinositol 3‐kinase (PI3K)/Akt pathway inhibitor) (0, 5, 10, and 20 μ m ). Proliferation was evaluated in CD8+ and CD4+ T cells. (D) Quantified percentages of proliferating CD8+ and CD4+ T cells from (C). Data represent mean ± SEM from three independent experiments ( n = 3). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Journal: FEBS Open Bio

Article Title: Indoleamine 2,3‐dioxygenase 1 inhibition reverses cancer‐associated fibroblast‐mediated immunosuppression in high‐grade serous ovarian cancer

doi: 10.1002/2211-5463.70126

Figure Lengend Snippet: IDO1 inhibition promotes T‐cell proliferation by increasing protein kinase B (Akt) phosphorylation suppressed by CAFs. (A) Flow cytometric analysis of phosphorylated Akt (ser473) in PBMCs. Cells were activated with OKT3 (15 ng·mL −1 ) in the presence or absence of OV05 CAFs at a PBMC:CAF ratio 6:1 and with or without epacadostat (500 n m ) for 5 days. Histograms show p‐Akt expression in CD8+ and CD4+ T cells. (B) Quantified p‐Akt levels in CD8+ and CD4+ T cells from experiments using three different CAFs (OV01, OV02, and OV05). (C) Flow cytometry histograms show T‐cell proliferation (CFSE stained) with increasing concentrations of LY294002 (phosphatidylinositol 3‐kinase (PI3K)/Akt pathway inhibitor) (0, 5, 10, and 20 μ m ). Proliferation was evaluated in CD8+ and CD4+ T cells. (D) Quantified percentages of proliferating CD8+ and CD4+ T cells from (C). Data represent mean ± SEM from three independent experiments ( n = 3). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Techniques: Inhibition, Phospho-proteomics, Expressing, Flow Cytometry, Staining, Comparison

IDO1 inhibition restores T‐cell cytotoxicity suppressed by CAFs in ovarian cancer cells. (A) Flow cytometric analysis of CAOV3 ovarian cancer apoptosis under different coculture conditions. CAOV3 cells were cultured alone (top left), with OKT3‐activated PBMCs (15 ng·mL −1 , 5 days) (top right), with activated PBMCs and CAFs (bottom left), or with activated PBMCs, CAFs, and epacadostat (500 n m , 5 days) (bottom right). Apoptosis was assessed by FITC‐Annexin V and PI staining. Early apoptotic (Annexin V+ PI−) and late apoptotic/necrotic (Annexin V+ PI+) cell percentages are shown in quadrants. (B) Quantified apoptotic CAOV3 cells under different treatment conditions. Bars indicate mean apoptotic cell percentages (Annexin V+) from experiments using three CAFs (OV01, OV02, and OV05). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Journal: FEBS Open Bio

Article Title: Indoleamine 2,3‐dioxygenase 1 inhibition reverses cancer‐associated fibroblast‐mediated immunosuppression in high‐grade serous ovarian cancer

doi: 10.1002/2211-5463.70126

Figure Lengend Snippet: IDO1 inhibition restores T‐cell cytotoxicity suppressed by CAFs in ovarian cancer cells. (A) Flow cytometric analysis of CAOV3 ovarian cancer apoptosis under different coculture conditions. CAOV3 cells were cultured alone (top left), with OKT3‐activated PBMCs (15 ng·mL −1 , 5 days) (top right), with activated PBMCs and CAFs (bottom left), or with activated PBMCs, CAFs, and epacadostat (500 n m , 5 days) (bottom right). Apoptosis was assessed by FITC‐Annexin V and PI staining. Early apoptotic (Annexin V+ PI−) and late apoptotic/necrotic (Annexin V+ PI+) cell percentages are shown in quadrants. (B) Quantified apoptotic CAOV3 cells under different treatment conditions. Bars indicate mean apoptotic cell percentages (Annexin V+) from experiments using three CAFs (OV01, OV02, and OV05). Statistical significance was determined using one‐way ANOVA followed by Tukey's multiple comparison tests (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns = not significant).

Techniques: Inhibition, Cell Culture, Staining, Comparison

IDO1 expression was related to poor prognosis in HCC. ( A) Representative images of IDO1 immunofluorescence staining of HCC tissue and paracancerous tissue in patients with HCC and quantitative results of the fluorescence intensity of IDO1. ( B ) Kaplan–Meier curves for OS in HCC patients based on IDO1expression level. ( C ) The IDO1 expression grouped by age, gender ( D ), tumor size ( E ), stage ( F ), AFP ( G ), HBV ( H ), cirrhosis ( I ), metastasis ( J ), relapse ( K ) and HBcAb ( L ). The data were presented as mean ± SEM (* P < 0.05).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 expression was related to poor prognosis in HCC. ( A) Representative images of IDO1 immunofluorescence staining of HCC tissue and paracancerous tissue in patients with HCC and quantitative results of the fluorescence intensity of IDO1. ( B ) Kaplan–Meier curves for OS in HCC patients based on IDO1expression level. ( C ) The IDO1 expression grouped by age, gender ( D ), tumor size ( E ), stage ( F ), AFP ( G ), HBV ( H ), cirrhosis ( I ), metastasis ( J ), relapse ( K ) and HBcAb ( L ). The data were presented as mean ± SEM (* P < 0.05).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

The infiltration and prognostic significance of mature DCs subsets in HCC. ( A ) Representative images of immunofluorescence staining of CK, CD11C, MHCII, CD40, CD80, in cancer and paracancerous tissues from HCC patients. ( B ) Quantitative analysis of the percentage of CD11C + , CD11C + MHCII + , CD11C + CD80 + and CD11C + CD40 + DCs. ( C ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + DCs. ( D ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + MHCII + DCs. ( E ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD80 + DCs. ( F ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD40 + DCs. ( G ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + MHCII + DCs, according to Spearman correlation analysis. ( H ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD80 + DCs, according to Spearman correlation analysis. ( I ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD40 + DCs, according to Spearman correlation analysis. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: The infiltration and prognostic significance of mature DCs subsets in HCC. ( A ) Representative images of immunofluorescence staining of CK, CD11C, MHCII, CD40, CD80, in cancer and paracancerous tissues from HCC patients. ( B ) Quantitative analysis of the percentage of CD11C + , CD11C + MHCII + , CD11C + CD80 + and CD11C + CD40 + DCs. ( C ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + DCs. ( D ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + MHCII + DCs. ( E ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD80 + DCs. ( F ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD40 + DCs. ( G ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + MHCII + DCs, according to Spearman correlation analysis. ( H ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD80 + DCs, according to Spearman correlation analysis. ( I ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD40 + DCs, according to Spearman correlation analysis. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Immunofluorescence, Staining

IDO1 inhibition could attenuate the EMT and malignant proliferation of SK-HEP1 in vitro. ( A ) Western blot analysis was used to detect the expression levels of Vimentin and IDO1 in control and experimental SK-HEP1 cells at 24 h after EPA intervention. GAPDH served as a loading control. The density of protein was measured using Quantity One software. ( B ) Western blot analysis was used to detect the expression levels of E-cadherin and PCNA in control and experimental SK-HEP1 cells at 24 h after EPA intervention. α-tubulin served as a loading control. The density of protein was measured using Quantity One software. ( C ) Relative mRNA levels of IDO1, E-cadherin, Vimentin, and PCNA were measured in SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could attenuate the EMT and malignant proliferation of SK-HEP1 in vitro. ( A ) Western blot analysis was used to detect the expression levels of Vimentin and IDO1 in control and experimental SK-HEP1 cells at 24 h after EPA intervention. GAPDH served as a loading control. The density of protein was measured using Quantity One software. ( B ) Western blot analysis was used to detect the expression levels of E-cadherin and PCNA in control and experimental SK-HEP1 cells at 24 h after EPA intervention. α-tubulin served as a loading control. The density of protein was measured using Quantity One software. ( C ) Relative mRNA levels of IDO1, E-cadherin, Vimentin, and PCNA were measured in SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vitro, Western Blot, Expressing, Control, Software

IDO1 inhibition could improve the malignant biological behavior of SK-HEP1 cells in vitro. ( A ) The proliferation of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was evaluated using the Key Fluor 488 Click-iT Edu assay. ( B ) The proliferation rate of SK-HEP1cells in ( A ) was quantified in a bar graph. ( C ) The cell viability of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was assessed using the CCK-8 assay. The cell viability reported as fold change of EPA-treated vs control cells. ( D ) Representative images from the transwell assay (left) and migrating cells number analysis (right). ( E ) Representative images from the wound healing assay. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could improve the malignant biological behavior of SK-HEP1 cells in vitro. ( A ) The proliferation of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was evaluated using the Key Fluor 488 Click-iT Edu assay. ( B ) The proliferation rate of SK-HEP1cells in ( A ) was quantified in a bar graph. ( C ) The cell viability of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was assessed using the CCK-8 assay. The cell viability reported as fold change of EPA-treated vs control cells. ( D ) Representative images from the transwell assay (left) and migrating cells number analysis (right). ( E ) Representative images from the wound healing assay. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vitro, EdU Assay, CCK-8 Assay, Control, Transwell Assay, Wound Healing Assay

IDO1 inhibition could delay the subcutaneous tumor formation of SK-HEP-1 cells in vivo. ( A ) Schematic protocol for animal experiment. ( B ) The tumor growth curve of mice and the representative diagrams of tumors in each group. ( C ) Tumor weight of the mice in control and EPA-treated group. ( D ) Immunofluorescence staining of IDO1 in tumor tissues in control and EPA-treated group. ( E ) The serum levels of Trp and Kyn were determined in mice from both the control and EPA-treated groups. ( F ) HE staining of tumor tissues in control and EPA-treated group. ( G ) Immunohistochemistry staining of PCNA in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could delay the subcutaneous tumor formation of SK-HEP-1 cells in vivo. ( A ) Schematic protocol for animal experiment. ( B ) The tumor growth curve of mice and the representative diagrams of tumors in each group. ( C ) Tumor weight of the mice in control and EPA-treated group. ( D ) Immunofluorescence staining of IDO1 in tumor tissues in control and EPA-treated group. ( E ) The serum levels of Trp and Kyn were determined in mice from both the control and EPA-treated groups. ( F ) HE staining of tumor tissues in control and EPA-treated group. ( G ) Immunohistochemistry staining of PCNA in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vivo, Control, Immunofluorescence, Staining, Immunohistochemistry

IDO1 inhibition could enhance immune cells response to tumor cells in vivo. ( A ) Immunofluorescence staining of CD11C, MHCII,CD80 and CD40 in tumor tissues in control and EPA-treated group. ( B ) Immunofluorescence staining of CD4 and CD8 in tumor tissues in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could enhance immune cells response to tumor cells in vivo. ( A ) Immunofluorescence staining of CD11C, MHCII,CD80 and CD40 in tumor tissues in control and EPA-treated group. ( B ) Immunofluorescence staining of CD4 and CD8 in tumor tissues in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vivo, Immunofluorescence, Staining, Control

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